Journal: Biomolecules & Therapeutics
Article Title: Combination of Cannabidiol with Taurine Synergistically Treated Periodontitis in Rats
doi: 10.4062/biomolther.2024.104
Figure Lengend Snippet: Inhibitory effect on osteoclast differentiation and bone resorption. (A) RAW264.7 cells were cultured in DMEM high glucose for 5 days in the presence of RANKL (50 ng/mL) with CBD, taurine, and both. (B) RAW264.7 cells were seeded in CaP-coated 48-well plates and treated with 50 ng/mL RANKL in the presence of CBD, taurine, and both. (C) TRAP-positive multinucleated cells (TRAP+ MNCs) were counted after fixation and staining for TRAP. (D) Pit area was measured. The results are presented as means ± SD (n=3). *** p <0.001 indicates significant differences from CBD+taurine treated groups with CBD and taurine alone.
Article Snippet: The bone resorption pit assay was carried out using a Bone Resorption Test Kit (CSF-BRA-48 KIT, Cosmo Bio) in plates coated with calcium phosphate.
Techniques: Cell Culture, Staining